Deeper imaging. Deeper insight. Deeper understanding. That's the new Dragonfly 600!
ANDOR 's Dragonfly 600 spinning disk confocal system is a high-contrast, multidimensional imaging platform that, with its unmatched combination of speed and sensitivity, enables researchers to discover unforeseen dynamic events and image living organisms for days at a time. This new model builds on the successful Dragonfly 500 series and focuses primarily on super-resolution DNA-PAINT localization imaging and high-resolution 3D tissue imaging. With its flexible scripting interface, the Dragonfly 600 can be further combined with fluidic systems for large-scale multi-omics imaging.
Like its predecessors, the Dragonfly 500 and 200, the new Dragonfly 600 offers four key imaging modalities - fast spinning disk confocal imaging, laser widefield imaging, transmitted light imaging (DIC, phase contrast, brightfield) and new modules for super-resolution microscopy (3D SMLM and B-TIRF™).
Dragonfly provides excellent multidimensional images from subcellular samples (on the nm scale) to the whole organism (on the cm scale). New super-resolution modules and the patented B-TIRF reveal even the smallest details, such as the dynamics of viral infection or the ultrastructure of chromatin or organelles.
All this is possible thanks to three key technological innovations:
- The HLE-700 - a laser engine with 2-7 multimode laser lines in a single chassis (up to 10 lines in a dual configuration) providing the high powers (from hundreds of mW to units of Watt in the 400-785 nm range) needed to image single molecules. A technical note on the HLE laser engine can be read here
- B-TIRF™ - the new Borealis™ TIRF module for super-resolution and single molecule imaging, taking advantage of the uniform sample illumination and power density provided by the patented Borealis™ system
- 3D-SMLM - 3D super-resolution module provides parfocal astigmatic encoding for super-resolution in all imaging modalities (widefield, confocal, B-TIRF)
Instrument control and data acquisition is provided by the Fusion user software with an API (REST) programmable in Python, MATLAB, LabVIEW, etc. REST enables protocol sequencing that can be used for multiplexed labeling applications. The localization analysis is performed by the Picasso tool, which was developed as an open-source program in the Jungmann lab. Picasso reads and writes data obtained in the IMARIS analysis software (in IMS format) and localization data in HDF5 format. Imaris 9.9 analysis software supports HDF5 for 3D visualization of localization and voxelized data.
Fusion, Imaris and Picasso work in harmony.
Read more about this new flagship system from ANDOR in the datasheet or on the product page here.