Confocal Raman microscope with FLIM module

The client requested a confocal Raman microscope with computer-controlled adjustment of the physical confocal aperture in the range of at least 25 µm to 100 µm. The instrument had to provide spectral resolution <0.1 cm^-1 and spatial resolution had to be <1 µm. The powerful spectrograph had to have a focal length of ≥225 mm. It had to include at least two integrated lasers – 532 nm and 785 nm. The system also had to be equipped with a fluorescence module for mapping fluorescence lifetime using TCSPC technology in the range of 230-850 nm and a module for temperature control of the sample in the range of at least -120 to 600 °C.

For this task, we chose the RMS1000 confocal Raman microscope (manufactured by Edinburgh Instruments) with spectral resolution <0.1 cm^-1 and spatial resolution <1 µm.

The setup includes the following components:

1. Fully automated Raman up-right microscope with computer-controlled physical confocal aperture settings (more than 10 different sizes in the range of 25 µm to 100 µm)
2. Powerful spectrograph with a focal length of 225 mm
3. Integrated 532 nm and 785 nm laser sources
4. Fluorescence module for fluorescence lifetime mapping using TCSPC technique; including 2 picosecond pulsed lasers (448 nm and 375 nm)
5. Sample holder with computer-controlled temperature in the range of -190 to 600 °C
6. Software for controlling the entire system (Raman spectrum mapping, FLIM, etc.) and data processing