Special MicAO 3DSR module with adaptive optics

One of the world's leading manufacturers of wave analyzers and adaptive optics, the French company Imagine Optic, has developed a special MicAO 3DSR module with adaptive optics, specifically with the HASO4 First Shack-Hartmann sensor and the Mirao 52e deformable mirror, to improve the quality of single-molecule methods in all three dimensions by up to 40%. The MicAO 3DSR module can correct for astigmatism and aberrations of the optical system, allowing more photons to be detected from the sample and thus contributing to increased resolution and localization accuracy in superresolution localization methods (PALM, STORM, SPT).

Key features of this module include:

• Best 3D localization accuracy
• increase in the number of detected photons up to two times
• correction of spherical aberrations of the optical system
• control of the scattering function (PSF) of the optical assembly and restoration of the axial symmetry of the PSF
• Compatibility with 60X, 100X lenses and FOV of most sCMOS cameras
• Ability to perform single-molecule imaging in deeper layers (up to 50 µm) of water-based samples
• Wave analyzer stable for more than 12 hours
• PSF reproducibility

This module has been developed for easy implementation to any inverted microscope, where the device is simply inserted between the microscope side port and the scanning camera. The MicAO 3DSR is supplied together with the user-friendly MicAO v1.3 software, which contains all the necessary tools to detect and correct aberrations in biological samples.