It involves compositing the captured image data into a single resulting high-resolution image that covers a much larger area than the single field of view of the camera. The image data is captured on the microscope by shifting the field of view and the subsequent fusion is made possible by special software. The data can be fused very accurately and smoothly based on the microscope stage position information stored in the metadata (in case the stage position information is missing, the individual images have to be manually aligned). With the software, 2D, 3D and 4D data up to 1 Tb can be stacked.
