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Dragonfly 200 confocal microscope

Sales of these systems have been discontinued.

The Dragonfly 200 confocal microscope is the ideal solution for visualizing living cells or organisms - live cell imaging Live cell microscopy, or live cell imaging, is a popular method in the field of cell biology.

Due to the high scanning speed and extreme sensitivity of the detectors, the toxic effects of light and bleaching fluorophores are reduced. The acquisition time for a high-quality image is minimal compared to scanning confocal microscopes (LSCM), which is an advantage not only when fast processes need to be captured, but also for routine spatial imaging of fixed samples.

Key features

Fast and gentle confocal microscope - Spinning disk
Resolution of 2048 × 2048pixels and more - SRRF Stream
Wide field imaging by extending the disk - Widefield
Integrated Borealis sample perfect illumination system
Complementary software with deconvolution and super-resolution- Fusion
Image analysis and 3D visualizationsoftware - Imaris Core

Unparalleled speed and sensitivity of imaging is due to the use of state-of-the-art high quantum efficiency camerasfrom Andorand the patented Borealis illumination system. The Dragonflyconfocal microscope is complemented by the new, complementary Fusion software. Also included is the basic version of Bitplane's excellent image analysis and 3D visualization software, Imaris Core.

The Dragonfly 200 confocal microscopeprovides even more flexibilityregarding the microscope body. Both direct and inverted microscopesfrom the world's leading manufacturers are compatible. The technology behind the excellent performance is identical for both systems. For detailed information on the differences in configuration, please refer to the technical specification below.

ANDOR

Aplikační oblasti:

Microscopes

Parameters

Confocal imaging speed	103 fps (2048 × 2048 pixels, Zyla 4.2 Plus camera)
Disc slit size	25 μm, 40 μm
Disc rotation speed	6000 rpm
Minimum exposure	2.5 ms
Wavelength range (Confocal/Widefield imaging)	Excitation: 400-800 nm Emission: 420-850 nm
Wavelength range (TIRF imaging)	400-640 nm
Optical zoom at illumination (Borealis)	1×, 2×, 4×, 6×
Optical zoom for camera	1×, 1.5×, 2×

Accessories

Fusion

Complementary software for Dragonfly systemcontrol and basic image data processing.

User-friendly interface with intuitive controls
Real-time3D visualization simultaneously with scanning
GPU accelerateddeconvolution - ClearView
Super-resolution microscopy - SRRF Stream
Sample positioning on the microscope ( software joystick x, y, z)
Check and adjust optical path parameters

Imaris Core

The core version of the Imarisanalysis software ( Bitplane ) builds on Fusion with a common data format *.ims, simplifying and speeding up the transition between image data acquisition and analysis. For more information, please visit the Imaris product page.

3D image and time-lapse 4D imageanalysis
Annotation and surfacerepresentation
Easy creation of illustrative video sequences
3D visualization and display of optical sections

Cameras

For maximum scanning speed, the confocal microscope comes with a Zyla 4.2 / ZL41 Cell or SONA ( sCMOS) camera .

For maximum sensitivity, we recommend the iXon Ultra 888 ( EMCCD) camera with a quantum efficiency greater than 90%.

Dragonfly can be fitted with two cameras in different combinations to allow simultaneous sensing of two channels, or use a camera with more appropriate specifications for a particular application.

Borealis

Patented illumination system for SDCM Abbreviation for Spinning Disc Confocal Microscopy.
show in dictionary (Spinning disc confocal microscopy)

Uniform illumination of the entire field of view
Greater light beamtransmission
Extended excitation spectrum (NIR imaging)
Improved axial geometry
More stable pairing with the light source (Laser)

Integrated lighting system for widefield and confocal imaging. The laser beam is guided by a multi-vision optical fiber into a so-called Beam Conditioning Unit where the beam is homogenized. This beam is then guided by another multi-vision optical fiber to the beginning of the optical path where it serves as a light source for imaging. Download the full application note on Borealis here.