MicAO 3DSR

show in dictionary (Photo Activation Localization Microscopy), STORM (Stochastic Optical Reconstruction Microscopy), or stochastic optical reconstruction microscopy, is one of the methods of super-resolution microscopy using light flashes to activate photoactivatable fluorescent molecules. The photoactivation of molecules occurs based on their stochastic (random) properties.
Show in glossary (Stochastic Optical Reconstruction Microscopy) and SPT (Single Particle Tracking).

The principle of the MicAO 3DSR module is based on the use of adaptive optics, specifically a Mirao 52e deformable mirror correcting for astigmatism and aberrations of the optical system, which allows the detection of multiple photons from a sample. This contributes to overcoming the diffraction limitations of microscopy imaging and leads to increased resolution accuracy of SMLM methods.

The MicAO 3DSR has been developed for easy implementation on any inverted microscope, where the module is simply inserted between the microscope side port and the camera itself, allowing the use of additional add-on systems. The MicAO 3DSR is supplied together with the user-friendly MicAO v1.3 software, which contains all the necessary tools for aberration detection and correction in biological samples.