The instrumentation of the service laboratory of light microscopy at the Institute of Molecular Genetics of the CAS, which is part of the Czech-BioImaging research infrastructure, has recently been expanded by a multi-modalconfocal microscope Dragonfly with Mosaic3 module for photo-manipulation and optogenetics. The microscope is equipped with a sophisticated incubation system for imaging objects under controlled conditions. The introduction of fast confocal imaging technology using the rotating disk technique significantly expands the possibilities of the service laboratory users, especially in the field of imaging living cells or organisms in the embryonic state of development.
Description of the new equipment:
The confocal microscope consists of a Dragonfly module configured with two cameras: the Zyla 4.2 Plus and the iXon Ultra 888, which also allow simultaneous dual-channel imaging. The system is equipped with two laser modules with six lasers providing excitation in the spectral region from 405nm to 640nm. The fully motorised Leica DMI8 microscope body is equipped with a wide range of high quality objectives and is complemented by a Mosaic3 module for FRAP, optogenetics and other photo-stimulation applications. The microscope is equipped with a chamber incubator for temperature and humidity control. In addition, an incubation chamber for perfect control of gas concentration (CO2/O2). The entire system is controlled by a powerful workstation equipped with Fusion software with image deconvolution capability and Imaris software for immediate 3D visualization.
Components:
- Andor Dragonfly - Confocal microscope
- Andor Zyla 4.2 Plus - sCMOS camera
- Andor iXon Ultra 888 - EMCCD camera
- Leica DMI8 - Microscope body
- TMC Micro-g - Optical stage
- Andor Mosaic 3 - Photo-stimulation module
- OKOLab Bold Line - Incubator
- Andor Fusion - acquisition software with deconvolution
- Bitplane Imaris Core - 3D visualization software
